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Shiitake Mushroom Research

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Click here to view the full Shiitake Mushroom study in "Journal of Biochemistry and Molecular Biology", Vol. 35, No. 5, September 2002.

Construction of a Genetic Linkage Map of Shiitake Mushroom Lentinula Edodes Strain L-54

From fruiting bodies of L. edodes strain L-54, single-spore isolates (SSIs) were collected. Two parental types of L-54 were regenerated via monokaryotization. By means of random-amplified polymorphic DNA (RAPD), DNA samples from L-54, its two parental types, and 32 SSIs were amplified with arbitrary primers. Dedikaryotization was demonstrated, and 91 RAPD-based molecular markers were generated. RAPD markers that were segregated at a 1:1 ratio were used to construct a linkage map of L. edodes.

This RAPD-linkage map greatly enhanced the mapping of other inheritable and stable markers [such as those that are linked to a phenotype (the mating type), a known gene (priA) and a sequenced DNA fragment (MAT)] with the aid of mating tests, bulked-segregant analysis, and PCR-single strand conformational polymorphism. These markers comprised a genetic map of L. edodes with 14 linkage groups and a total length of 622.4 cM.

Keywords: Genetic map, Genetic mapping, Lentinula edodes, Random-amplified polymorphic DNA (RAPD)

Click here to view the full study from the Journal of Biochemistry and Molecular Biology, Vol. 35, No. 5, September 2002, pp. 465-471